We are extremely fortunate to be supported by the University of California, the National Institutes of Health (NIH) and private funding sources. With this amazing support we are able to purchase the following equipment. All members of our group learn to use each of these techniques for their projects.
Leica Infinity TIRF super-resolution microscope equipped with an X-Cite 120 LED light source with filters for CFP, YFP, GFP and mCherry, four laser lines (405 nm, 488 nm, 561 nm and 647 nm) with matched penetration depth, simultaneous imaging capabilities, and optogenetic activation. Additionally, the system has a 60x 1.45 NA TIRF, 100x 1.49, and 163x 1.49 NA TIRF oil immersion objectives, a Hamamatsu orca flash 4.0 camera, and a fully isolated stage-top incubator to permit long-term imaging at physiological temperature with 95% O2 and 5%CO2.
Andor W1-spinning Disk confocal with a Borealis modification, built around an Olympus IX83 inverted microscope base and equipped with four laser lines (445 nm, 488 nm, 514 nm and 561 nm), a Cairn Optosplit III (to allow simultaneous imaging of up to three channels), a TIRF module to permit 2 channel sequential TIRF, and a fully isolated stage-top incubator to permit long-term imaging at physiological temperature with 95% O2 and 5%CO2.
Zeiss LSM 880 (with Airy scan) confocal microscope paired with a HEKA patch clamp system
The Dickson lab electrophysiological & Imaging suite is also home to an Olympus IX83 Cell TIRF microscope equipped with an X-Cite 120 LED light source with filters for CFP, YFP, GFP and mCherry, four laser lines (445 nm, 488 nm, 514 nm and 561 nm) with matched penetration depth capabilities, a 60x 1.49 NA TIRF, oil immersion objective, a 100x 1.49 NA TIRF, oil immersion objective, an Andor iXon Ultra 888 back thinned EMCCD camera and a Cairn Optosplit III.
The Dickson lab is also home to a Nikon Sweptfield confocal built around a Nikon Eclipse TE 2000-S inverted microscope equipped with a Photometrics Quantem 512SC EMCCD camera, 100x 1.49 NA TIRF oil, 60x 1.49 NA TIRF oil, 60x 1.2 NA water, 40x 1,3 NA oil, 20x and 10x objectives and two laser lines (488 and 561 nm).